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Representative immunohistochemistry images of SEMA6A in SKCM tissues and normal skin tissues (HPA) (a) SEMA6A expression level, ∗ represents p < 0.05 (b). The results of quantitative reverse transcription PCR (RT-qPCR) of SEMA6A expression level in SKCM cell lines (c) ∗∗∗, ∗∗, and ∗ represent significant differences compared with human primary normal skin melanocytes ( p < 0.001, p < 0.01, and p < 0.05), respectively.

Journal: International Journal of Genomics

Article Title: Comprehensive Analysis to Identify LINC00511–hsa-miR-625-5p–SEMA6A Pathway Fuels Progression of Skin Cutaneous Melanoma

doi: 10.1155/2023/6422941

Figure Lengend Snippet: Representative immunohistochemistry images of SEMA6A in SKCM tissues and normal skin tissues (HPA) (a) SEMA6A expression level, ∗ represents p < 0.05 (b). The results of quantitative reverse transcription PCR (RT-qPCR) of SEMA6A expression level in SKCM cell lines (c) ∗∗∗, ∗∗, and ∗ represent significant differences compared with human primary normal skin melanocytes ( p < 0.001, p < 0.01, and p < 0.05), respectively.

Article Snippet: Based on the manufacturer's instructions, the study involved the extraction of total RNAs from both melanoma cell lines and human primary normal skin melanocyte cell lines using TRIzol (Takara Biomedical Technology (Beijing) Co., Ltd., Beijing, China).

Techniques: Immunohistochemistry, Expressing, Quantitative RT-PCR

Exploration of upstream LncRNAs/pseudogenes of hsa-miR-625-5p in SKCM and their network. (a) Network diagram representing the upstream lncRNAs and pseudogenes of hsa-miR-625-5p in SKCM. The diamond shape represents lncRNAs, and the ellipse represents pseudogenes. (b) Analysis of expression levels and correlation of LINC00240 with SKCM. (c) Analysis of expression levels and correlation of LINC00511 with SKCM. (d) Analysis of expression levels and correlation of MPRIPP1 with SKCM. (e) Analysis of expression levels and correlation of LINC00909 with SKCM. (f) Analysis of expression levels and correlation of UBQLN4P1 with SKCM. (g) Analysis of expression levels and correlation of PVT1 with SKCM. (h) Results of RT-qPCR for LINC00511 expression levels in SKCM cell lines. (i) Results of RT-qPCR for UBQLN4P1 expression levels in SKCM cell lines. Note . ∗, ∗∗, and ∗∗∗ represent significant differences compared with human primary normal skin melanocytes ( p < 0.05, p<0.01, and p < 0.001), respectively.

Journal: International Journal of Genomics

Article Title: Comprehensive Analysis to Identify LINC00511–hsa-miR-625-5p–SEMA6A Pathway Fuels Progression of Skin Cutaneous Melanoma

doi: 10.1155/2023/6422941

Figure Lengend Snippet: Exploration of upstream LncRNAs/pseudogenes of hsa-miR-625-5p in SKCM and their network. (a) Network diagram representing the upstream lncRNAs and pseudogenes of hsa-miR-625-5p in SKCM. The diamond shape represents lncRNAs, and the ellipse represents pseudogenes. (b) Analysis of expression levels and correlation of LINC00240 with SKCM. (c) Analysis of expression levels and correlation of LINC00511 with SKCM. (d) Analysis of expression levels and correlation of MPRIPP1 with SKCM. (e) Analysis of expression levels and correlation of LINC00909 with SKCM. (f) Analysis of expression levels and correlation of UBQLN4P1 with SKCM. (g) Analysis of expression levels and correlation of PVT1 with SKCM. (h) Results of RT-qPCR for LINC00511 expression levels in SKCM cell lines. (i) Results of RT-qPCR for UBQLN4P1 expression levels in SKCM cell lines. Note . ∗, ∗∗, and ∗∗∗ represent significant differences compared with human primary normal skin melanocytes ( p < 0.05, p<0.01, and p < 0.001), respectively.

Article Snippet: Based on the manufacturer's instructions, the study involved the extraction of total RNAs from both melanoma cell lines and human primary normal skin melanocyte cell lines using TRIzol (Takara Biomedical Technology (Beijing) Co., Ltd., Beijing, China).

Techniques: Expressing, Quantitative RT-PCR